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ATTACHMENT A SCOPE OF SERVICES 'Continuation of monitoring comparing fished and unfished reefs on an Artificial Reef East Coast Demonstration Project (Indian River County) ' Introduction This task is a 3'd year continuation of artificial reef monitoring that began under F42 Segment 15 on a set of artificial reefs which were constructed under Project # 1 , Task #2 of F42-Segment 14 , as a Florida Atlantic Coast cooperative demonstration project . The project is intended to demonstrate to the public the potential habitat and fisheries resource enhancement value of artificial reefs under conditions where there is reduced fishing pressure . This is the final year of the three year monitoring project and has the sponsorship of a local county government, a university with a marine biology department, and a local fishing club . Under the current scenario , two of four reefs existing in three permitted sites will have unpublished locations for three years. The two remaining reefs will be fished . The four reef sites were permitted to Indian River County under ACOE Permit # 199700975 (GP-TB ) . The approximate coordinates of the patch reefs are : North Latitude West Longitude Depth Permitted Site #2 270 55 . 941 ' 800 15 . 941 ' 66 feet Patch Reef 2NW Permitted Site #3 270 54 . 733' 800 13 . 710' 71 feet Patch Reef 3NW Permitted Site #4 270 55 . 537 ' 800 12 . 733 ' 73 feet Patch Reef 4NW Permitted Site #4 270 54 . 679' 800 11 . 863 ' 72 feet Patch Reef 4SE Fish populations will continue to be censused on all four reefs (eventually covering a 36 month period ). Each of the four reefs will be surveyed at least two (two) times during this agreement period . For each survey, a pair of divers will collect two sets of data . One diver will use a JVC underwater digital video camera to record images of fishes along a path extending an estimated 3 m on either side of each transect line (designated T1 , T2 , and T3) , while the second diver will use a slate to record presence/absence of all species seen , including those not directly on the transects . Each dive will began with a survey along the complete perimeter ( P) , followed by surveys along T1 - T3 . A second survey along T2 will be conducted , with special attention to fishes inhabiting the deepest holes and crevices in the reef. Repeated surveys of T1 and T3 will be performed if possible , within the 30 minute air/bottom time limitation of working at depths of 70 feet. The data collected will allow for calculation of parameters such as species diversity and community diversity. Size estimates will be collected on two to four target fish species , and laser scale guides in videography will allow for calibration of the diver size estimates . Data will also be collected on the degree of fishing effort in order to calculate changes in catch per unit effort (CPUE ) . User survey forms have been developed and distributed . Data analysis using statistical methods will be difficult due to the small sample size , so it is proposed that if the assumptions of an Analysis of Variance (ANOVA) such as homogeneity and normality of the data can be met , then a one-way ANOVA with repeated measures will be used to test for differences in Page I of 4 , Attachment A