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PERMITTEE: Indian River County u alities PERMIT NUMBER FL0041637 1840 25th Street EXPIRATION DATE: July 22, 2004 Vero Beach, FL 32960 IRCUD/West Regional WWTF 1 . a. The permittee shall conduct 96-hour acute static renewal toxicity tests using the daphnid, Ceriodanhnia duhij and the bannerfm shiner, Cvnrinella leedsi. All tests will be conducted on four separate grab samples collected at evenly-spaced (6-hr) intervals over a 24-hour period and used in four separate tests in order to catch any peaks of toxicity and to account for daily variations in effluent quality. b. If control mortality exceeds 10% for either species in any test, the test(s) for that species (including the control) shall be repeated. A test will be considered valid only if control mortality does not exceed 10% for either species . If, in any separate grab sample test, 100% mortality occurs prior to the end of the test, and control mortality is less than 10% at that time, that test (including the control) shall be terminated with the conclusion that the sample demonstrates unacceptable acute toxicity. 2. a. The toxicity tests specified above shall be conducted once every two months only during natural discharge events until 4 valid tests are completed. These tests are referred to as "routine" tests. Upon the completion of four valid tests which demonstrate that no unacceptable toxicity (as defined in 4.a.) has been identified, the Department shall review the data and , if approval is granted, shall provide written permission to reduce the toxicity monitoring frequency to annually. b . Results from `routine" tests shallbe reported according to EPA/600/4=90/027F, Section 12, Report Preparation (or the most current edition), and shall be submitted to: Florida Department of Environmental Protection Central District Office 3319 Maguire Boulevard Suite 232 Orlando, Florida 32803 =3767 3 . a. All "routine" tests shall be conducted using a control (0% effluent) and test concentration of 100% final effluent, b. Mortalities of equal to or greater than 50% in a 100% effluent routine sample or an LC50 equal to or less than 100% effluent calculated during any follow-up definitive test will constitute a violation of these permit conditions, and Rule 62-302 .200( 1 ), Rule 62-302.500( 1)(d) and, Rule 624.244(3)(a), F. A. C. 4. a. If unacceptable acute toxicity (greater than 20% mortality of either test species in any of the four separate grab sample tests within the specified time) is found in a `routine" test, the permittee shall conduct two additional tests on the specie(s) indicating unacceptable toxicity. The first additional test will include four grab samples taken as described in La. and run as four separate definitive analyses. The second additional definitive test will be run on a single grab sample collected on the day and time when the greatest toxicity was identified in the first additional definitive test. Results for each additional test will include the determination of LC50 values with 95% confidence limits. b. The first additional test shall be conducted using a control (0% effluent) and a minimum of five dilutions : 100%9 50%0 25%, 12.5% and 6.25% effluent and a control (0% effluent). The dilution series may be modified in the second test to more accurately identify the toxicity, such that at least two dilutions above and two dilutions below the target toxicity and a control (0% effluent) are run. c. For each additional test, the sample collection requirements and the test acceptability criteria specified in Section 1 above must be met for the test to be considered valid. The first test shall begin within two weeks of the end of the `routine" tests, and shall be conducted weekly thereafter until two additional, valid tests are completed. The additional tests will be used to determine if the toxicity found in the `routine" test is still present. d. Results from additional tests, required due to unacceptable acute toxicity in the "routine" tests, shall be submitted in a single report prepared according to EPA/600/4=90/027F, Section 12, or the most current edition and submitted within 45 days of completion of the additional, valid tests . If the additional test(s) demonstrate unacceptable acute toxicity, the permittee will meet with the Department within 30 days of the report submittal to identify corrective actions necessary to remedy the unacceptable acute toxicity. 7 D.E.P. - ORLANDO EXI] IBIT NO, 2