Laserfiche WebLink
„Nu•„h4 4 <br /> , <br /> (DME)technique. DME analysis by EMSL Analytical, Inc. of the tape lift <br /> samples collected by Engineering Systems, Inc. (EMSL Analytical, Inc., Test <br /> Reports of Microscopic Examination of Fungal Spores,Fungal Structures, <br /> Hyphae, and Other Particulates from Tape Samples, analysis date April 17, 2015) <br /> and DME analysis by EMLab P&K of the swab samples collected by AMEC <br /> Environment& Infrastructure, Inc. (EMLab P&K, Direct Microscopic <br /> Examination Report, reports dated April 6,2015) show the growth of mold genera <br /> Stachybotrys, Cladosporium, and Penicillium. The growth of these mold genera <br /> indicates that sufficient moisture was present on the sampled surfaces and caused <br /> the actual growth. <br /> 3. Culturing of swab samples collected from surfaces is an incorrect way of <br /> establishing mold growth. <br /> Sampling of surfaces with swabs will pick up settled mold spores from all <br /> kinds of indoor and outdoor environments. The collected mold spores, if viable, <br /> will grow with the nutrients the Petri dishes provide. This does not mean that the <br /> presence of just mold spores is mold growth. <br /> The results from EMSL Analytical, Inc. for swab cultures(EMSL <br /> Analytical,Inc., Test Report of Identification and Enumeration of Culturable <br /> Fungi by Swab, Station 1,analysis date April 15, 2015)reported Aspergillus <br /> creber and Penicillium chrysogenum growth in Petri dish. The analytical <br /> technique does not indicate if these two species were actually growing on the <br /> sampled surfaces. Furthermore, culturing of swabs greatly exaggerates the <br /> results. It is well known that the process of culturing, and in particular the <br /> dilution process,has a disadvantage because of increase in bias associated with a <br /> dilution factor. When a sample is taken, it is often diluted in series to reduce <br /> overcrowding of Colony Forming Unit(CFU); therefore,a culture plate that has <br /> the lowest dilution(e.g. 1:10) will have the highest concentration of CFU and the <br /> plates that have highest dilutions(e.g. 1:10,000)will have the lowest <br /> concentration of CFU. The formation of only 34 colony counts will give 340,000 <br /> 73 <br />