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hardbottom, sediment over benthos (where the underlying fauna cannot be identified <br /> due to the sediment cover) , and sand . <br /> The remainder of the 150-meter video transect shall be reviewed for qualitative changes <br /> in benthic community cover/composition in comparison to the pre-construction surveys, <br /> previous annual surveys, and the reference sites. If the qualitative review of the video <br /> transect suggests sedimentation impacts or cross-shore transport of sediments over the <br /> benthic community along the remaining portions of the transect, and the results of the <br /> sedimentation monitoring and quadrat assessments indicate burial of hardbottom <br /> communities, additional quantitative analysis of the video transects shall be required as <br /> directed by the Department to determine the extent of the project-related impacts. <br /> Quadrat sampling <br /> During each survey , a 0.25 square meter gridded quadrat will be sampled by the <br /> diver/biologist at a several point-intercept locations along the 150-meter long transects. <br /> The first quadrat will be located at the nearshore hardbottom edge . If the hardbottom <br /> edge consists of a vertical ledge, one quadrat will be located along the vertical/sub- <br /> vertical face, a second quadrat will be located at the top of the ledge along at the <br /> horizontal face of the ledge, and the third quadrat shall be placed at the 5-meter mark <br /> along the transect. Additional quadrat sampling shall occur along each transect to <br /> capture changes in benthic landscape (physical relief, species dominance/abundance, <br /> presence of stony corals/octocorals along or immediate adjacent to the transect) . If the <br /> transect crosses a sand gap (i.e. trough) , a quadrat shall be sampled at the seaward <br /> edge of the exposed hardbottom , and at the west edge of hardbottom east of the sand <br /> gap (i . e. on the horizontal face of the crest of the next ledge). The distance across the <br /> sand gap shall be noted and is included within the 150-meter length of the transect. <br /> Quadrats shall not be placed in the sand gaps between hardbottom ridges. Each 150- <br /> meter transect shall have approximately 9-10 quadrats. A list of the locations of the <br /> quadrats along each transect with a description of the quadrat location/benthic <br /> community shall be provided to the Department after transect establishment. <br /> Parameters for Quadrat Sampling <br /> Within each quadrat, visual estimate of percent cover and genus/species identification <br /> shall be performed in-situ for the following functional groups: macroalgae (overall <br /> percent cover and identification/percent cover of two dominant species within quadrat), <br /> microalgae/cyanobacteria, encrusting calcareous algae, sponges (genus level) , with a <br /> separate assessment of percent cover of boring sponge (Cliona spp) , tunicates (with <br /> identification of dominant genera), zoanthids (genus level), hydroids, wormrock <br /> (Phragmatopoma lapidosa) , octocorals (genus level) , and scleractinian corals (species <br /> level) . Individual counts shall be conducted for all octocorals, scleractinian corals, <br /> sponges (not including Cliona spp. which shall be assessed for percent cover) , solitary <br /> tunicates, urchins, and holothuroids within the quadrat. <br /> Scleractinian corals and octocorals shall also be assessed for size class distribution : <br /> size class 1 : < 5 cm diameter of scleractinian/height of octocoral ; size class 2 : 5 cm to <br /> 15 cm diameter of scleractinian/height of octocoral; size class 3: 15 to 25 cm diameter of <br /> scleractinian/height of octocoral; and size Gass 4: >25 cm diameter of <br /> scleractinian/height of octocoral) . Maximum and average algal height of the two <br /> C1Do eft ar WhVsyona wgIL=1 Settlrgl% wrwy kte RmXOIK2M2 sox FPlAI.d <br /> 3 <br />