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ATTACHMENT A SCOPE OF SERVICES Monitoring of Artificial Reefs Located in Indian River Count Introduction This task is an expansion and enhancement of artificial reef monitoring that began under F42 Segment 15 on a set of artificial reefs which were constructed under Project # 1 , Task #2 of F42-Segment 14 , as a Florida Atlantic Coast cooperative demonstration project . Four reef sites were permitted to Indian River County under ACOE Permit #199700975 ( P -TB). The approximate coordinates of the . railroad tie patch reefs. are : North Latitude West Longitude Depth Permitted Site #2 270- 55 . 941 ' 80 ° 15 . 941 ' 66 feet Patch Reef 2NW Permitted Site #3 270 54 . 733 ' 80° 13 . 710 ' 71 feet Patch Reef 3NW Permitted Site #4 270 55 . 537' 800 12 . 733 ' 73 feet Patch Reef 4NW Permitted Site #4 27° 54 . 679 ' 80011 , 8631 72 feet Patch Reef 4SE Fish populations will continue to be censused on all four reefs . Each of the four reefs will be surveyed at least four times during this agreement period (twice in the Spring April-June 2005 , and twice in the fall September- October 2005) , for a total of 16 survey events . For each survey, a pair of divers will collect two sets of data . One diver will use a JVC underwater digital video camera to record images of fishes along a path extending an estimated 3 m on either side of each transect line ( designated T1 , T2 , and T3) , while the second diver will use a slate to .record presence/absence of all species seen , including those not directly on the tmc nsects . Each dive will began with a survey along the complete perimeter (P) , followed by surveys along T1 - T3 . A , second survey along T2 will be conducted , with special attention to fishes inhabiting the deepest holes and crevi es in the reef. Repeated surveys of T1 and T3 will be performed if possible , within the 30 minute air/bottom time limitation of working at depths of 70 feet. The data collected will allow for calculation of parameters such as species diversity and community diversity. Size estimates will be collected on fish species of recreational importance , and laser scale guides in videography will allow for calibration of the diver size estimates . ' Data will also be collected on the degree of fishing effort observed at the reef sites during each sampling day. Data analysis using statistical methods will be difficult due to the small sample size , so it is roposed that if the assumptions of an Analysis of Variance (ANOVA) such as homogeneity and normality of the data can be met, then a one-way ANOVA with repeated measures will be used to test for differences in species abundance over time . If ANOVA assumptions cannot be met, an analogous non-parametric method will be used (Weiner 1971 ) . Variability of the fish assemblages over time will be evaluated by comparing the mean coefficient of variation for all species at control and treatment reefs ( Seber 1973 ) , The 'T' method for multiple comparisons among pairs of means (Zar 1984) will test for significant differences in fish community variability. The reef monitoring to be funded consists of the following elements : 1 . Approximate Proiect Timeline a . April-June 2005 : Spring Monitoring and Data Collection (Quantitative Assessment) Page /8 of 14 '7 '�"