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species abundance over time . If ANOVA assumptions cannot be met, an analogous non -parametric <br /> method will be used (Weiner 1971 ) . Variability of the fish assemblages over time will be evaluated by <br /> comparing the mean coefficient of variation for all species at control and treatment reefs (Seber 1973 ) . <br /> The 'T' method for multiple comparisons among pairs of means (Zar 1984 ) will test for significant <br /> differences in fish community variability. <br /> Community parameters that do not depend on sample size such as the Bray-Curtis index of similarity <br /> (Clifford and Stephenson 1975 ) will test the hypothesis that the fish populations of the two treatment reefs <br /> are no more similar to each other than they are to the control reefs . The Shannon-Weiner species <br /> diversity index will be used to compare species richness and evenness . <br /> The reef monitoring to be funded consists of the following elements : <br /> 1 . Approximate Project Timeline <br /> a . March-May 2003 : Monitoring and Data Collection (Quantitative Assessment) <br /> b . May 2003 : Data Entry, Summary and Analysis <br /> C , June 2003 : Reporting <br /> 2 , Survey Methodology <br /> a. Survey methodology will collect reliable data on patterns of community structure, <br /> relative abundance, and size of resident fishes . <br /> b . Data will be collected from 4 sites during a 3 month sampling window. The total number <br /> of monitoring events will include a minimum of 8 fish census events (2 replicates at each site) . <br /> i . Fish surveys will be conducted at each site by implementing an in situ visual <br /> survey method along the fixed transect, supplemented by a videographic visual survey <br /> method along the fixed transect. Surveys will be conducted at two separate intervals during <br /> this study. <br /> C , If visibility is judged to be less than 15 feet horizontally on the bottom, no fish census <br /> will be performed and the dives to conduct said surveys will be repeated at a later date . <br /> 3 . Data Analysis <br /> a. Analysis shall include a comparison of similarity and differences in patterns of <br /> community structure, relative abundance, and size of resident fishes for the following: <br /> i . Unfished reefs (reefs 2NW and 4NW) ; and <br /> ii . Fished Reefs (reefs 3NW and 4SE) . <br /> 4 . Deliverables <br /> a . Written or electronically submitted status reports will be submitted every 30 days : <br /> March 30, 2003 , April 30, 2003 , and May 30, 2003 . <br /> b . A draft report will be submitted to the Commission by June 1 , 2003 to provide 21 days <br /> for technical peer review by the Commission . <br /> Page 2 of 4, Attachment A <br />
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