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2006-285
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2006-285
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Within each quadrat, visual estimate of percent cover and genus/species identification <br /> shall be performed in-situ for the following functional groups: macroalgae (overall <br /> percent cover and identification/percent cover of two dominant species within quadrat), <br /> microalgae/cyanobacteria, encrusting calcareous algae, sponges (genus level), with a <br /> separate assessment of percent cover of boring sponge (Cliona spp), tunicates (with <br /> identification of dominant genera), zoanthids (genus level), hydroids, sabeliariid worm <br /> (Phragmatopoma lapidosa), octocorals (genus level), and scleractinian corals (species <br /> level). Individual counts shall be conducted for all octocorals, scleractinian corals, <br /> sponges (not including Cliona spp. which shall be assessed for percent cover), solitary <br /> tunicates, urchins, and holothuroids within the quadrat. <br /> Scleractinian corals and octocorals shall also be assessed for size class distribution: size <br /> class 1 : < 5 cm diameter of scleractinian/height of octocoral; size class 2 : 5 cm to 15 cm <br /> diameter of scleractinian/height of octocoral ; size class 3 : 15 to 25 cm diameter of <br /> scleractinian/height of octocoral; and size class 4 : >25 cm diameter of <br /> scleractinian/height of octocoral). Maximum and average algal height of the two <br /> dominant macroalgal species shall also be recorded. Average algal height shall be <br /> estimated by five measurements . <br /> Percent cover of substrate, unconsolidated substrate (rubble, shell hash, rhodoliths), sand <br /> and sediment over hardbottom shall be recorded. The maximum physical relief of <br /> hardbottom from the lowest point to highest point in the quadrat, shall be measured to the <br /> nearest centimeter. Sediment accumulation measurements will be performed within all <br /> quadrats located on the fiat (horizontal) surface of hardbottom along each transect. Five <br /> random measurements of sediment thickness will be performed within each quadrat using - <br /> a ruler to measure to the nearest cm. Continuous layers of sediment less than 1 cm <br /> covering the hardbottom surface and benthos shall be recorded . <br /> Benthic Data Analysis <br /> The methodology for monitoring the benthic community succession on the mitigation <br /> artificial reef is identical to the monitoring of the natural hardbottom for the <br /> renourishment of Sectors 1 and 2 . The artificial reef benthic communities will be <br /> compared to the natural hardbottom communities for each reef type (high relief and low <br /> relief) using community parameters such as percent cover, species richness, species <br /> dominance, sediment cover, average algal height, and substrate categories. These <br /> parameters will be compared between the artificial reef and natural reef for each reef type <br /> by one-way ANOVA tests. Data will be transformed as necessary to meet ANOVA <br /> assumptions and non-parametric tests (Mann-Whitney U test, Wilcoxon Rank-Sum) will _ <br /> be used on the original diversity data if the data do not distribute normally after <br /> transformation. To detect differences between the artificial reef and natural reef within <br /> each reef type, the Bray-Curtis similarity index based on the percent cover will be used. <br /> If there is dominance by a few, highly abundant taxa; a log transformation will be used to <br /> reduce their contribution to similarity and to strengthen the contribution of the less <br /> common taxa. <br /> 4 <br />
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