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Hydro rice South Relief Canal Algal Turf Scrubber® Pilot Program • RO Concentrate will be sampled biweekly as grab samples for bioassay associated with the shrimp Mysidopsis bahia • RO Concentrate will be sampled as grab samples at project initiation , mid - term , and project end for bioassay associate with Menidia berrylina . • The ATSTm effluent will be sampled bi -weekly for three samplings as a minimum , as a grab sample , for acute toxicity testing using bioassay with the species Pimephales promelas (fathead minnow) and Ceriodaphnia dubia (daphnia ) . • The ATSTm effluent will be sampled bi -weekly for three samplings as a minimum , as composite samples , for chronic toxicity testing using bioassay with the species Pimephales promelas (fathead minnow) and Ceriodaphnia dubia (daphnia) . This chronic testing will be initiated only after three consecutive acute tests determined to meet FDEP criteria as non -toxic . • Algal turf biomass will be harvested periodically (typically every week or every other week) . The harvested biomass will be weighed in the field , and solids content determined from composited grab samples for each harvest . These dried composite samples will be combined and analyzed as monthly composites for nutrient content . • Flow meters will be placed upon the RO concentrate flow ( by Utilities Department) , the South Canal flow ( HydroMentia ) and the effluent flow ( HydroMentia ) . Totalized flows will be recorded weekly . Influent flows will be calculated as the sum of the two flow meter totalizers over the same time period . • A rain gauge will be placed on site and recording documented weekly , along with air temperature . • In - Situ pH , conductivity/salinity , DO and water temperature will be taken weekly using a YSI -590 ( or equivalent) handheld unit . Plan of Study 8