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a <br /> will be located at monuments R- 113 and R- 115 . Final transect positions will be adjusted <br /> to maximize the amount of hard bottom habitat along each transect . <br /> A total of 17 photoquadrat [0 . 5 m square (0 .25m2)] stations will be positioned along each <br /> transect. Photoquadrat stations will be positioned at 5 m intervals from the western end <br /> of the transect to the 50-m mark ( 11 quadrats) and at 60 , 70, 80 , 90 , 140 , and 180 m <br /> marks . Stainless steel pins with an attached numbered tag shall be installed at the <br /> above-described intervals and used for placement of the lower left comer of the quadrat, <br /> with all quadrats placed alongside and to the south of the transect. Stainless steel rods <br /> ( 1 . 25 cm diameter by 75 cm length) will be installed with at least 50 cm visible above <br /> the seafloor at the nearshore and offshore end of each transect and in areas of low-relief <br /> hard bottom at approximately 20 m and 50 m along the transect. These rods will not be <br /> placed in areas of high vertical relief (ledges) . They will be used to measure sediment <br /> accumulation along the transects. <br /> Within the quadrats the following parameters will be measured and estimated in situ: <br /> 1 . Percent sediment cover within the quadrat; <br /> 2 . Sediment thickness (maximum thickness - will be measured up to 30 cm ; <br /> average sediment thickness - will be measured at 10 intersections on the 10-cm <br /> quadrat grid ; a continuous layer of sediments of < 1 cm in the matrix of micro- or <br /> macroalgae will be recorded as < 1 cm) ; <br /> 3 . Maximum relief of hard bottom (recorded as difference in height from the lowest <br /> point of hard bottom to the highest point of hard bottom within the quadrat) ; <br /> 4 . Percent cover by dominant groups of benthic organisms (leafy algae , encrusting <br /> algae , sponges , hydroids, Phragmatopoma , stony corals, bryozoans, tunicates) <br /> using the 10-cm grids within the quadrat frame ; <br /> 5 . Individual counts and taxonomic identification for stony corals , octocorals, <br /> sponges , tunicates, and urchins. Maximum dimensions will be recorded for all <br /> stony corals , octocorals, and sponges; <br /> 6 . Maximum and average (by five haphazard measurements) algal thallium (algal <br /> height) ; <br /> 7 . Percent cover of algae, broken down into dominant species (or at least to genus- <br /> level) ; and <br /> 8. Digital photograph of the entire quadrat. <br /> Up to 12 additional quadrats will be established along the 12 transects for the monitoring <br /> of individual large colonies of stony corals ( Oculina varicosa , O. robusta , Siderastrea <br /> siderea , etc. ) which are visible from the transect during station installation . These <br /> colonies will be marked with a numbered tag attached to a stainless steel pin epoxied <br /> into the bottom adjacent to each colony . The health status of each colony along with <br /> size, possible sand burial , and/or presence of sand around each colony within the <br /> quadrat frame will be recorded , and a photograph will be taken during each survey . <br /> Quadrat photographs will be analyzed to calculate percent cover of algae and attached <br /> biota using Point Count software, a random point percent cover analysis program . <br /> Species richness , total percent cover, and community structure (rank orders of species <br /> abundance) will be compared between transects (and between surveys following <br /> subsequent sampling efforts) . The percent cover results from the post-survey random <br /> point analysis will be compared to the in situ diver estimates of percent cover. If results <br /> CADoe mens and SettingsyonethangUcal SettingsUemporary Internet FIIesXOLK2EWrnendment 13 scope.doc 7 <br />