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( 1 ) Americamysis (Mysidopsis) bahia routine toxicity testing shall be <br /> conducted monthly, the first starting within 30 days of the issuance of this Consent Order and <br /> lasting for the duration of this Consent Order. <br /> (2) Menidia beryllina routine toxicity testing shall be conducted quarterly <br /> concurrent with a monthly Americamysis (Mysidopsis) bahia routine toxicity test. The first <br /> Menidia beryllina test shall start within 60 days of issuance of this Consent Order and lasting for <br /> the duration of this Consent Order. <br /> c . Sampling Requirements : <br /> ( 1 ) Routine tests shall be conducted on four separate grab samples collected at <br /> evenly-spaced ( 6-hr) intervals over a 24-hour period. The four grab samples shall be used in four <br /> bioassays for each species to be tested and shall represent one test. If the duration of the discharge is <br /> less than 24-hours , the duration of discharge shall be documented on the chain of custody. <br /> Sufficient sample at each sampling event shall be collected for chemical analysis of ionic imbalance <br /> ions including potassium, calcium, sodium, magnesium, chloride, bromide , and sulfate and for a <br /> Major Seawater Ion Imbalance Toxicity (MSIIT) Test . The chemical analysis is to be performed on <br /> each sample collected . The MSIIT is to be started immediately using the same effluent sample when <br /> an Americamysis (Mysidopsis) bahia bioassay has greater than 50 percent mortality in the 100% <br /> effluent dilution at or prior to 96 hours from test initiation . The MSIIT test is conducted following <br /> the protocols contained in "Technical Guidance Document for Conducting the Florida Department <br /> of Environmental Protection ' s Protocols for Determining Major- Seawater-Ion Imbalance Toxicity <br /> (MSIIT) in Membrane-Technology Water-Treatment Concentration" , FDEP , Bureau of <br /> Laboratories , May 4 , 2004 . A copy of the protocol may be downloaded from the Department ' s <br /> Web Site . If multiple grab samples from the same sampling event have greater than 50% mortality <br /> in the 100% effluent, the MSIIT needs to be conducted on one of the grab samples , not all four. <br /> 5 of 17 OGC File No . 08- 1661 <br />