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(2) Upon successfully completing the Major Seawater Ionic Imbalance Toxicity test, and following discussion of the results with the Department as provided in 9 . f. (6) , the Respondent may request a reduction in test frequency for the Americamysis (Mysidopsis) bahia bioassays from monthly to quarterly. All reductions in test frequency must be approved by the Department prior to implementation . d. Test Requirements : ( 1 ) All routine tests shall be conducted using a control (0% effluent) and a minimum of five dilutions : 100 % , 80 % , 40 % , 20 % , and 10 % effluent . (2) The permittee shall conduct 96-hour acute static renewal toxicity tests using the mysid, Americamysis (Mysidopsis) bahia , and the inland silverside, Menidia beryllina . (3 ) All test species , procedures and quality assurance criteria used shall be in accordance with Methods for Measurine Acute Toxicity of Effluents and Receivine Waters to Freshwater and Marine Organisms, EPA- 821 -R-02 -012 . Any deviation of the bioassay procedures outlined herein shall be submitted in writing to the Department for review and approval prior to use . In the event the above method is revised, the permittee shall conduct acute toxicity testing in accordance with the revised method. (4) The control water and dilution water used shall be artificial seawater and diluted to the test salinity as described in EPA-821 -R-02 -012 , Section 7 . 2 . 4 . The test salinity shall be determined as follows : (a) When the salinity of the effluent is between 1 and 7 parts per thousand (ppt) , the following salinity adjustment shall be used. For the Americamysis bahia bioassays , the effluent and the control (0% effluent) shall be adjusted to a salinity of 7 ppt for the 100% effluent test using artificial sea salts . No salinity adjustment shall be done for the Menidia beryllina bioassay test. The salinity of the control/dilution water (0% effluent) shall match the test salinity of the effluent. A 6 of 17 OGC File No . 08 - 1661