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salinity adjustment control should be prepared and included with the Americamysis bahia bioassay . The salinity adjustment control is intended to identify toxicity resulting from adjusting the salinity of the effluent with artificial sea salts . To prepare the salinity adjustment control , dilute the control/dilution water to the salinity of the effluent and adjust the salinity of the salinity adjustment control to 7 ppt at the same time that the salinity of the effluent is adjusted to 7 ppt, using the same artificial sea salts . (b) When the salinity of the effluent is greater than 7 ppt, no salinity adjustment shall be made to the effluent and the test shall be run at the effluent salinity . The salinity of the control/dilution water (0% effluent) shall match the test salinity of the effluent. e . Quality Assurance Requirements : ( 1 ) A standard reference toxicant ( SRT) quality assurance (QA) acute toxicity test shall be conducted with each species used in the required toxicity tests either concurrently or initiated no more than 30 days before the date of each routine or additional follow-up test conducted. The SRT test must be conducted concurrently if the test organisms are obtained from outside the test laboratory unless the test organism supplier provides control chart data from at least the last five monthly acute toxicity tests using the same reference toxicant and . test conditions . If the organism supplier provides the required SRT data, the organism supplier ' s SRT data and the test laboratory ' s monthly SRT-QA data shall be included in the reports for each companion routine or additional follow-up test required. (2 ) If the mortality in the control (0% effluent) exceeds 10% for either species in any test, the test for that species (including the control) shall be invalidated and the test repeated . The repeat test shall begin within 14 days after the last day of the invalid test . 7 of 17 CGC ile No . 08 - 156 i